Cuzzubbo et al (2001) Clin Diag Lab Immun

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Use of Recombinant Envelope Proteins for Serological Diagnosis of Dengue Virus Infection in an Immunochromatographic Assay Andrea J. Cuzzubbo,1, Timothy P. Endy,2 Ananda Nisalak,2 Siripen Kalayanarooj,3 David W. Vaughn,4 Steven A. Ogata,5 David E. Clements,5 and Peter L. Devine1,

(1) PanBio Ltd., Windsor 4030, Queensland, Australia (2) Department of Virology, Armed Forces Research Institute of Medical Sciences (3) Queen Sirikit National Institute for Child Health, Bangkok, Thailand (4) Department of Virus Diseases, Walter Reed Army Institute of Research, Silver Spring, Maryland (5) Hawaii Biotech, Inc, 99-193 Aiea Heights Dr., Suite 200 Aiea, HI 96701, USA

Clinical and Diagnostic Laboratory Immunology: Volume 8, No. 6, November 2001, Pages 1150-1155,

Abstract An immunochromatographic test that incorporates recombinant antigens (Dengue Duo Rapid Strip Test; PanBio, Brisbane, Australia) has recently become commercially available. This assay is performed in 15 min and detects both immunoglobulin M (IgM) and IgG in a capture format. The four recombi nant proteins used represent the Nterminal 80% of the viral envelope glycoproteins of dengue viruses 1, 2, 3, and 4, respectively. The sensitivity and specificity of the recombinant-antigen-based assay were 90 and 86%, respectively. The similar diagnostic performance of these antigens to that of enzyme-linked immunosorbent assays us ing whole dengue virus suggests that they mimic whole dengue viruse s in primary structure and epitope conformation. These results suggest that recombinant proteins can be used in diagnostic assays for dengue to overcome safety issues associated with the use of whole virus.

Link to full-text article: http://cdli.asm.org/cgi/content/full/8/6/1150?view=full&pmid=11687456